rh Interleukin-12 subunit p40

Human recombinant protein expressed in Nicotiana benthamiana.

RF0031

Molecular weight: rhuman Interleukin-12 p40 is a glycosylated  polypeptide chain containing 312 amino acids ( fragment  23 – 328 P29460 IL12B_HUMAN) with an amino- terminal  hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDSPAGE under reducing conditions.

Description: Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations.  The p40 subunit of IL-12 interacts with the β subunit of IL-12R and the disulfide linked p40 homodimer specifically antagonize the activity of bioactive IL-12 in different assay systems.

Biological Activity: the biological activity of IL12 p40 is determined by the dose dependent inhibition of IL-12 dependent IFN-gamma production (mouse splenocytes). Not available.

Figure 1. Western Blot analysis of recombinant IL12p40. Lane MWM:  Molecular weight marker (kDa), lane 1: 0.3 ug of IL12p40.

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